2XDY
Crystal structure of the N. crassa QDE-2 AGO MID domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.100, 91.390, 42.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.288 - 2.200 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.22290 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX (C/D) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.200 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 8285 | |
| <I/σ(I)> | 18.4 | 2.94 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 200 MM AMMONIUM SULFATE 16% PEG4000, pH 7.2 |
