2XDO
Structure of the Tetracycline degrading Monooxygenase TetX2 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.110, 80.270, 86.890 |
| Unit cell angles | 111.00, 90.23, 93.43 |
Refinement procedure
| Resolution | 40.540 - 2.090 |
| R-factor | 0.19192 |
| Rwork | 0.190 |
| R-free | 0.22993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SELENOMETHIONINE DERIVATIVE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.067 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.060 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 91733 | |
| <I/σ(I)> | 7.5 | 2 |
| Completeness [%] | 92.2 | 64 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.5 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 7 |
