2XDN
Transcription factor TtgR H67A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 43.111, 43.174, 114.803 |
Unit cell angles | 96.93, 99.26, 96.03 |
Refinement procedure
Resolution | 41.739 - 2.200 |
R-factor | 0.2219 |
Rwork | 0.218 |
R-free | 0.28790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.180 |
Data reduction software | MOSFLM |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.700 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.240 |
Number of reflections | 37118 | |
<I/σ(I)> | 7.8 | 2.9 |
Completeness [%] | 88.0 | 85.8 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |