2XDG
Crystal structure of the extracellular domain of human growth hormone releasing hormone receptor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 |
| Unit cell lengths | 31.648, 31.648, 287.138 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.410 - 1.950 |
| R-factor | 0.16742 |
| Rwork | 0.165 |
| R-free | 0.22288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x57 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.710 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.790 |
| Number of reflections | 11887 | |
| <I/σ(I)> | 14 | 2.6 |
| Completeness [%] | 97.6 | 95.9 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG6000, 0.15M MGCL2, 10% ETHYLENE GLYCOL, 0.1M TRIS PH7.0 |
