2XD0
A processed non-coding RNA regulates a bacterial antiviral system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.139, 119.125, 377.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 3.000 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEM MODEL OF TOXIN COMPLEX FROM SAME STUDY |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.642 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.320 |
| Number of reflections | 35628 | |
| <I/σ(I)> | 18 | 3.4 |
| Completeness [%] | 89.7 | 66.3 |
| Redundancy | 6.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.6M NACL, 0.1M MES PH6.5, 20% W/V PEG 4000 |
