2XCZ
Crystal Structure of macrophage migration inhibitory factor homologue from Prochlorococcus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-26 |
| Detector | MACSCIENCE DIP2030H |
| Spacegroup name | P 63 |
| Unit cell lengths | 52.450, 52.450, 63.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.220 - 1.640 |
| R-factor | 0.16604 |
| Rwork | 0.164 |
| R-free | 0.21277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1GIF 1gcz 1fim 1UIZ. |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.157 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.200 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.070 | 0.260 |
| Number of reflections | 12309 | |
| <I/σ(I)> | 15.3 | 6.4 |
| Completeness [%] | 98.7 | 91.3 |
| Redundancy | 5.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 42% PEG300, 0.2 M SODIUM CHLORIDE, 0.1 M HEPES PH 7.5, VAPOR DIFFUSION, HANGING DROP,TEMPERATURE 298 K |
