2XBP
A novel signal transduction protein PII variant from Synechococcus elongatus PCC7942 indicates a two-step process for NAGK PII complex formation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 80.682, 80.682, 80.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.080 - 1.200 |
| R-factor | 0.126 |
| Rwork | 0.125 |
| R-free | 0.14400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.734 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.090 | 1.060 |
| Number of reflections | 54696 | |
| <I/σ(I)> | 17.04 | 2.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 14 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PH 7 |
