2XB1
Crystal structure of the human Pygo2 PHD finger in complex with the B9L HD1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.750, 69.730, 82.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.310 - 1.900 |
| R-factor | 0.17441 |
| Rwork | 0.171 |
| R-free | 0.21715 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vp7 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.599 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.750 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.358 | |
| Number of reflections | 18032 | |
| <I/σ(I)> | 6.4 | 1.6 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6% PEG 1000, 1.6 M (NH4)2SO4, 100 MM HEPES PH 7.5, 45 MM HCL |
