2XAE
Crystal structure of human kinesin Eg5 in complex with (R)-2-amino-3-((S)-2-methyl-1,1-diphenylbutylthio)propanoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 96.770, 96.770, 124.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.18512 |
| Rwork | 0.181 |
| R-free | 0.25708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wog |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.832 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 40212 | |
| <I/σ(I)> | 14.2 | 4.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 24% POLYETHYLENE GLYCOL-3350, 0.25 M AMMONIUM SULPHATE, 0.01 M TRIMETHYLAMINE HYDROCHLORIDE AND 0.1 M MES PH 6.0 |
