Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-26 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.253, 68.253, 179.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.760 - 2.340 |
| R-factor | 0.23826 |
| Rwork | 0.234 |
| R-free | 0.27974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.447 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.700 | 2.390 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 9121 | |
| <I/σ(I)> | 9.7 | 1.78 |
| Completeness [%] | 98.0 | 76 |
| Redundancy | 4.48 | 3.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 0.002ML PROTEIN/COMPLEX (0.050 ML TETR(D) (C = 17.3 MG/ML)50 ML 2MM ISO-CLTC) PLUS ML BUFFER (50 MM TRIS PH 8, 1.1 M (NH4)2SO4, 100 MM NACL, 10 MM MGCL2) HANGING DROP AGAINST 0.5 ML BUFFER |
