2X7Q

The conserved Candida albicans CA3427 gene product defines a new family of proteins exhibiting the generic periplasmic binding protein structural fold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-03
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	41.411, 65.724, 128.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.887 - 2.000
R-factor	0.2186
Rwork	0.218
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x7p
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.200	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.260
Number of reflections	17895
<I/σ(I)>	6.5	2.7
Completeness [%]	76.7	72.6
Redundancy	8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1