2X7P
The Conserved Candida albicans CA3427 Gene Product Defines a New Family of Proteins Exhibiting the Generic Periplasmic Binding Protein Structural Fold
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Collection date | 2004-07-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979774, 0.979958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.588, 66.849, 113.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.880 - 2.340 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.385 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.880 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 13442 | |
| <I/σ(I)> | 4.1 | 3 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 5.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 21 % PEG8000, CALCIUM ACETATE 0.2 M, TRIS 0.1 M, 30 % GLYCEROL PH 7.0 |
