2X77
Crystal Structure of Leishmania major ADP ribosylation factor-like 1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2009-05-14 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.286, 78.944, 58.034 |
Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
Resolution | 78.940 - 2.100 |
R-factor | 0.21773 |
Rwork | 0.214 |
R-free | 0.28682 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1moz |
RMSD bond length | 0.016 |
RMSD bond angle | 1.815 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.100 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.490 |
Number of reflections | 18516 | |
<I/σ(I)> | 11.5 | 2.4 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROPS OF 0.75 UL PROTEIN SOLUTION, 0.75 UL 75 MM CALCIUM ACETATE, 17 % PEG 3350. |