2X6S
Human foamy virus integrase - catalytic core. Magnesium-bound structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.350, 89.190, 177.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 2.290 |
| R-factor | 0.22757 |
| Rwork | 0.225 |
| R-free | 0.27807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x74 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.590 | 2.410 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.070 | 0.540 |
| Number of reflections | 59482 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 97.4 | 89.3 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8-2M AMMONIUM FORMATE, 100MM HEPES, PH 7.5 5MM MGCL2, 10-15% GLYCEROL |
