2X6O
Tet Repressor class D in complex with 7-chlor-2-cyano-iso- tetracycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 69.050, 69.050, 180.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.430 - 2.300 |
| R-factor | 0.21954 |
| Rwork | 0.214 |
| R-free | 0.26867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vke |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.430 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.260 |
| Number of reflections | 10063 | |
| <I/σ(I)> | 41.3 | 10.9 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 11.45 | 7.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 50 MM TRIS/HCL PH 9.5, 750 MM (NH4)2SO4, 70 MM NACL, 1 MM EDTA |
