2X6A

Potassium Channel from Magnetospirillum Magnetotacticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-16
Detector	ADSC CCD
Spacegroup name	P 4 21 2
Unit cell lengths	107.759, 107.759, 89.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.621 - 3.100
R-factor	0.2226
Rwork	0.220
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wlh
RMSD bond length	0.014
RMSD bond angle	1.108
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.140	0.960
Number of reflections	10032
<I/σ(I)>	20.1	2.9
Completeness [%]	100.0	100
Redundancy	13.8	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		SITTING DROP VAPOUR DIFFUSION, 2:1 PROTEIN:CRYSTALLANT RATIO. CRYSTALLANT: 25% PEG400 2.5% PEG4K 2.5% PEG8K 10% GLYCEROL 90MM HEPES PH 7.5 ADDITIVES: FOS-CHOLINE-ISO-11-6U (ANATRACE) NONYL MALTOSIDE (ANATRACE) FOS-CHOLINE ISO-9 (ANATRACE)