2X69

X-ray Structure of Macrophage Inflammatory Protein-1 alpha polymer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	287
Detector technology	CCD
Collection date	2001-11-01
Detector	ADSC CCD
Spacegroup name	P 62 2 2
Unit cell lengths	181.541, 181.541, 76.845
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	157.220 - 2.650
R-factor	0.23483
Rwork	0.223
R-free	0.26461
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.099
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.080	0.460
Number of reflections	22235
<I/σ(I)>	40.7	5.2
Completeness [%]	99.9	100
Redundancy	10.6	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1