2X5Z
Crystal structure of T. maritima GDP-mannose pyrophosphorylase in complex with GDP-mannose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.227, 96.035, 217.122 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.19374 |
| Rwork | 0.191 |
| R-free | 0.24415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x5s |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.440 |
| Number of reflections | 24606 | |
| <I/σ(I)> | 24.4 | 4.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE PH 5.0, 30% (V/V) MPD, 20 MM CALCIUM CHLORIDE |
