2X5H
Crystal structure of the ORF131 L26M L51M double mutant from Sulfolobus islandicus rudivirus 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-26 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 37.940, 46.971, 48.976 |
Unit cell angles | 90.22, 95.05, 91.78 |
Refinement procedure
Resolution | 14.000 - 1.800 |
R-factor | 0.20282 |
Rwork | 0.200 |
R-free | 0.25715 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x5g |
RMSD bond length | 0.009 |
RMSD bond angle | 1.149 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.040 | 0.190 |
Number of reflections | 28543 | |
<I/σ(I)> | 24.8 | 6.5 |
Completeness [%] | 97.0 | 95 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2.1 M SODIUM MALONATE AND CRYOPROTECTED WITH 2.4 M MALONATE. |