2X4S
Crystal structure of MHC CLass I HLA-A2.1 bound to a peptide representing the epitope of the H5N1 (Avian Flu) Nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.377, 84.112, 80.035 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 19.926 - 2.550 |
| R-factor | 0.1818 |
| Rwork | 0.178 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.121 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 27072 | |
| <I/σ(I)> | 5.8 | 1.1 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
