2X4O
Crystal structure of MHC CLass I HLA-A2.1 bound to HIV-1 envelope peptide env120-128
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.988, 81.904, 79.748 |
| Unit cell angles | 90.00, 90.87, 90.00 |
Refinement procedure
| Resolution | 48.580 - 2.300 |
| R-factor | 0.1784 |
| Rwork | 0.175 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.426 |
| Data reduction software | MOSFLM |
| Data scaling software | TRUNCATE |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.920 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 35564 | |
| <I/σ(I)> | 6.3 | 5.24 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
