2X4N
Crystal structure of MHC CLass I HLA-A2.1 bound to residual fragments of a photocleavable peptide that is cleaved upon UV-light treatment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.509, 86.523, 80.003 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 19.806 - 2.340 |
| R-factor | 0.2004 |
| Rwork | 0.196 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.317 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.060 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 33198 | |
| <I/σ(I)> | 9.02 | 2.47 |
| Completeness [%] | 92.4 | 92.7 |
| Redundancy | 1.88 | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES PH 5.5, 20% PEG 1500. CRYSTALS WERE FROZEN IN 100 MM MES, 30% PEG 1500, 10% GLYCEROL |
