2X4I

ORF 114a from Sulfolobus islandicus rudivirus 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-13
Detector	ADSC QUANTUM 4
Spacegroup name	P 43
Unit cell lengths	48.790, 48.790, 195.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.080 - 2.200
R-factor	0.24319
Rwork	0.241
R-free	0.27736
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2BLK
RMSD bond length	0.008
RMSD bond angle	0.986
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.080	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.660
Number of reflections	16793
<I/σ(I)>	11.6	2.1
Completeness [%]	76.8	32.4
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		31% PEG1500, 0.1M HEPES, PH 7.5