2X34
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2008-07-23 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.979, 64.964, 84.191 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.430 - 1.900 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.22300 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.479 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.120 | 0.410 |
Number of reflections | 27856 | |
<I/σ(I)> | 14.4 | 5.5 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 42% PEG600, 0.2M IMIDAZOL-MALATE PH 5.5 |