2X32
Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-10-05 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.378, 64.360, 84.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.230 - 1.550 |
| R-factor | 0.16028 |
| Rwork | 0.159 |
| R-free | 0.19418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x34 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.872 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 51047 | |
| <I/σ(I)> | 17.1 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
