2X30
Crystal structure of the r139n mutant of a bifunctional enzyme pria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.600, 63.600, 102.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.520 - 1.950 |
| R-factor | 0.21816 |
| Rwork | 0.216 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vep |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.389 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | 0.650 |
| Number of reflections | 18061 | |
| <I/σ(I)> | 23 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.1 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 1.50 M AMMONIUM SULFATE AND 100 MM SODIUM CITRATE PH 4.8 |
