2X2V
Structural basis of a novel proton-coordination type in an F1Fo-ATP synthase rotor ring
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.180, 97.340, 121.240 |
| Unit cell angles | 90.00, 104.73, 90.00 |
Refinement procedure
| Resolution | 44.951 - 2.500 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wie |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.951 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.980 |
| Number of reflections | 57111 | |
| <I/σ(I)> | 11.7 | 1.62 |
| Completeness [%] | 98.8 | 92.8 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.3 | pH 4.3 |
