2X2R
Crystal structure of human kinesin Eg5 in complex with (R)-2-amino-3-((4-chlorophenyl)diphenylmethylthio)propanoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-05-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 96.350, 96.350, 124.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.411 - 2.200 |
| R-factor | 0.1746 |
| Rwork | 0.172 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x88 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.290 |
| Number of reflections | 65550 | |
| <I/σ(I)> | 14.3 | 6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25% PEG3350, 0.15M SODIUM TARTRATE DIBASIC DIHYDRATE, 0.1M MES PH6.0 |
