2X1V
Crystal Structure of the activating H-Ras I163F mutant in Costello Syndrome, bound to MG-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 63 |
| Unit cell lengths | 73.140, 73.140, 62.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.285 - 1.700 |
| R-factor | 0.1907 |
| Rwork | 0.190 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q21 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.090 |
| Number of reflections | 1054 | |
| <I/σ(I)> | 46.14 | 5.73 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15 | 6.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 18% POLYETHYLENE GLYCOL (PEG) 6000, 100 MM HEPES PH 7.4, 150 MM MGCL2. |
