2X1P
Gelsolin Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 55.948, 59.342, 81.651 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 19.510 - 1.100 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hcv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.750 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 204183 | |
| <I/σ(I)> | 23.6 | 3.08 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 7.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 1000, 100 MM PHOSPHATE/ CITRATE PH 4.4, 100 MM LISO4 |
