2X17
The X-ray structure of Ferritin from Pyrococcus furiosus loaded with Ag(I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RAYONIX |
| Spacegroup name | P 41 |
| Unit cell lengths | 157.681, 157.681, 246.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.26132 |
| Rwork | 0.260 |
| R-free | 0.28338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jd6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.744 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 107788 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 8.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.1-2.3 M AMMONIUM SULFATE, pH 7.5 |
