2X13
The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with ADP and 3phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.940, 91.440, 108.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.360 - 1.740 |
| R-factor | 0.18695 |
| Rwork | 0.185 |
| R-free | 0.21810 |
| Structure solution method | MIR |
| Starting model (for MR) | 2wzb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.411 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.080 | 0.260 |
| Number of reflections | 40002 | |
| <I/σ(I)> | 9.1 | 3 |
| Completeness [%] | 98.4 | 97 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS/TRIS PH 6.5 21% P2000MME |
