2X0K

Crystal structure of modular FAD synthetase from Corynebacterium ammoniagenes

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 3
Unit cell lengths	133.472, 133.472, 133.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.490 - 1.950
R-factor	0.20676
Rwork	0.204
R-free	0.23711
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.230	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.090	0.460
Number of reflections	57834
<I/σ(I)>	19.9	2.7
Completeness [%]	100.0	100
Redundancy	11.4	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		1.5M LI2SO4, 0.1 M HEPES-NAOH, PH 7.5
1		7.5		1.5M LI2SO4, 0.1 M HEPES-NAOH, PH 7.5