2X03
The X-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase Y253A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-17 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 317.095, 317.095, 82.976 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 158.550 - 2.300 |
| R-factor | 0.19612 |
| Rwork | 0.194 |
| R-free | 0.22757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wda |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.932 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.800 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 138108 | |
| <I/σ(I)> | 6.4 | 13.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.0M NA FORMATE WITH 20% GLYCEROL AS A CRYOPROTECTANT |
