2X02
CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10 AT 1.35 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.550, 96.850, 125.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.450 - 1.350 |
| R-factor | 0.16291 |
| Rwork | 0.162 |
| R-free | 0.18662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4f |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.040 | 0.360 |
| Number of reflections | 124977 | |
| <I/σ(I)> | 27.2 | 3.3 |
| Completeness [%] | 96.0 | 79 |
| Redundancy | 6.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.6M AS, 0.1M HEPES, PEG 400, DMSO, PH 7 |
