2WZO

The structure of the FYR domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	53.867, 53.867, 91.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.325 - 1.600
R-factor	0.1793
Rwork	0.178
R-free	0.20190
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.012
RMSD bond angle	1.391
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.600
High resolution limit [Å]	1.600	1.600
Rmerge	0.080	0.420
Number of reflections	20876
<I/σ(I)>	18.8	5.7
Completeness [%]	99.8	98.9
Redundancy	10.4	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		1.0M POTASSIUM/SODIUM TARTRATE, 100MM HEPES, PH7.5, AT 17OC. 20% GLYCEROL USED AS A CRYOPROTECTANT.