2WZJ
Catalytic and UBA domain of kinase MARK2/(Par-1) K82R, T208E double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-28 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 162.240, 205.578, 91.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.394 - 2.786 |
R-factor | 0.1819 |
Rwork | 0.179 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zmu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.138 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.070 | 0.690 |
Number of reflections | 76705 | |
<I/σ(I)> | 19.64 | 2.49 |
Completeness [%] | 99.4 | 97.7 |
Redundancy | 4.97 | 4.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 60% TACSIMATE PH 7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |