2WZ9
Crystal structure of the thioredoxin domain of human TXNL2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-25 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.630, 52.060, 58.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.600 - 1.550 |
R-factor | 0.156 |
Rwork | 0.154 |
R-free | 0.17900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2diy |
RMSD bond length | 0.017 |
RMSD bond angle | 1.578 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.470 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.090 | 0.580 |
Number of reflections | 19534 | |
<I/σ(I)> | 9.5 | 2.2 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.4 M AMMONIUM CITRATE, PH 7 |