2WZ8
Family 35 carbohydrate binding module from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.814, 52.914, 58.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.270 - 1.500 |
| R-factor | 0.1326 |
| Rwork | 0.130 |
| R-free | 0.17538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w1w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.457 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.120 |
| Number of reflections | 20322 | |
| <I/σ(I)> | 37.3 | 12.1 |
| Completeness [%] | 97.1 | 95.8 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM ACETATE, 30% PEG 3350 |
