2WYT
1.0 A resolution structure of L38V SOD1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.690, 67.345, 52.577 |
| Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.000 |
| R-factor | 0.12501 |
| Rwork | 0.124 |
| R-free | 0.14677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.120 | 0.360 |
| Number of reflections | 136867 | |
| <I/σ(I)> | 14 | 2.2 |
| Completeness [%] | 98.3 | 77.3 |
| Redundancy | 5.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.75 | 150 MM NACL, 2-3M AMMONIUM SULPHATE, 100 MM NA ACETATE BUFFER PH 4.75 |
