2WYS
High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-22 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9791, 0.9793, 0.9756 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 173.700, 173.700, 135.325 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.850 - 2.750 |
| R-factor | 0.18771 |
| Rwork | 0.185 |
| R-free | 0.23629 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.941 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (SOLVE RESOLVE) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.500 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.190 | 1.000 |
| Number of reflections | 61428 | |
| <I/σ(I)> | 13 | 1.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.6 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 292 | 1M KH2PO4 AT 292K |
