2WYM
Structure of a metallo-b-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2008-07-27 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.221, 178.212, 111.559 |
| Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.600 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wyl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.500 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | |
| Number of reflections | 60423 | |
| <I/σ(I)> | 10.4 | 3.19 |
| Completeness [%] | 91.5 | 70.5 |
| Redundancy | 8.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.2M LITHIUM CITRATE |
