2WYL
Apo structure of a metallo-b-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.610, 178.560, 112.420 |
| Unit cell angles | 90.00, 103.82, 90.00 |
Refinement procedure
| Resolution | 14.940 - 2.590 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.866 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.790 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.160 | |
| Number of reflections | 60575 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 97.5 | 74 |
| Redundancy | 8.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 200 MM LITHIUM CITRATE |
