2WXZ
Crystal structure of rat angiotensinogen in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.499, 49.539, 150.275 |
| Unit cell angles | 90.00, 90.84, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.283 |
| Rwork | 0.282 |
| R-free | 0.31000 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.929 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0099) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.600 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.220 | 1.040 |
| Number of reflections | 23736 | |
| <I/σ(I)> | 8 | 1.2 |
| Completeness [%] | 98.5 | 96.8 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 20-25% PEG3350, 0.2M MGSO4, 0.1M TRIS -HCL, PH7.8 |
