2WXZ

Crystal structure of rat angiotensinogen in C2 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-01
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	130.499, 49.539, 150.275
Unit cell angles	90.00, 90.84, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.283
Rwork	0.282
R-free	0.31000
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	0.929
Phasing software	PHASER
Refinement software	REFMAC (5.5.0099)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.220	1.040
Number of reflections	23736
<I/σ(I)>	8	1.2
Completeness [%]	98.5	96.8
Redundancy	4.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8		20-25% PEG3350, 0.2M MGSO4, 0.1M TRIS -HCL, PH7.8