2WXQ
The crystal structure of the murine class IA PI 3-kinase p110delta in complex with AS15.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.130, 63.860, 116.630 |
| Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
| Resolution | 65.940 - 2.700 |
| R-factor | 0.22673 |
| Rwork | 0.225 |
| R-free | 0.28623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rd0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.424 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.340 | 2.720 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 28229 | |
| <I/σ(I)> | 11.35 | 1.69 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.47 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 20% (V/V) GLYCEROL, 10% (W/V) PEG 4K, 30 MM NANO3, 30 MM NA2HPO4, 30 MM (NH4)2SO4, 100 MM IMIDAZOLE PH 6.8 |
