2WW4
a triclinic crystal form of E. coli 4-diphosphocytidyl-2C-methyl-D- erythritol kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.051, 50.124, 66.035 |
| Unit cell angles | 88.77, 69.65, 65.14 |
Refinement procedure
| Resolution | 24.890 - 2.000 |
| R-factor | 0.20047 |
| Rwork | 0.197 |
| R-free | 0.26224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oj4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.328 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0094) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 31885 | |
| <I/σ(I)> | 5.6 | 2.9 |
| Completeness [%] | 94.0 | 93.3 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
