2WW0
Structure of the Family GH92 Inverting Mannosidase BT3990 from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.810, 151.960, 218.619 |
| Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
| Resolution | 218.230 - 2.800 |
| R-factor | 0.18577 |
| Rwork | 0.184 |
| R-free | 0.21156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.254 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.600 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | 0.490 |
| Number of reflections | 169366 | |
| <I/σ(I)> | 9.7 | 2.9 |
| Completeness [%] | 98.0 | 95.2 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% W/V 6000 PEG, 0.2 M NH4CL, TRIS(HYDROXYMETHYL)AMINOMETHANE (PH 8.0) |
