2WVO
Structure of the HET-S N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.260, 95.260, 170.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.100 - 2.300 |
| R-factor | 0.22705 |
| Rwork | 0.225 |
| R-free | 0.25693 |
| Structure solution method | MIR |
| Starting model (for MR) | 2wvn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.570 |
| Number of reflections | 40556 | |
| <I/σ(I)> | 23.8 | 3.6 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 292 | CRYSTALLIZED AT 292K IN 50 MM HEPES PH 7.5, 4.0 M NACL |
