2WVN
Structure of the HET-s N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 122.660, 122.660, 122.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.780 - 2.620 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.770 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.060 | 0.463 |
| Number of reflections | 10044 | |
| <I/σ(I)> | 24.1 | 3.9 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 10.1 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 292 | CRYSTALLIZED AT 292K WITH 20 MM TRIS PH 8.0, 30% PEG4000 |
