2WVI
Crystal Structure of the N-terminal Domain of BubR1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-12 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.796, 62.796, 90.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.390 - 1.800 |
R-factor | 0.22373 |
Rwork | 0.222 |
R-free | 0.25986 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 1.685 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.620 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.110 | 0.510 |
Number of reflections | 19685 | |
<I/σ(I)> | 21 | 4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 15.6 | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 22.5% (W/V) PEG 8000, 0.17 M TRIS PH 8.0, 0.01 M PRASEODYMIUM(III) ACETATE |